REBASE - The restriction enzyme database. Maintained by the New England Biolabs, USA. WatCut - Provides restriction analysis, silent mutation scanning and SNP-RFLP analysis. Allows for utilization of all commercially available type II restriction enzymes. RestrictionMapper - Finds restriction enzyme cleavage sites in linear and circular DNA sequences. Provides also a virtual digest function. Graphical Codon Usage Analyzer - Displays the codon quality in two different ways, codon usage frequency or relative adaptiveness values. FAQ included. PlasMapper - Offers server generated plasmid maps and image files, from input DNA sequences up to 20,000 bp. Includes citation, and downloadable source code. Maintained by the University of Alberta, USA. NEBcutter - Provides a DNA sequence analysis to find restriction enzymes sites. Finds also non-overlapping open reading frames using the E.coli genetic code. Readseq - Converts amino acid and nucleotide sequence data formats including FASTA, GenBank, Phylip and others. Maintained by NIH, USA. Oligo Calc: Oligonucleotide Properties Calculator - Provides reverse complement sequence for DNA or RNA primer strands, self complementarity check, physical and thermodynamic constants, melting temperature (Tm). Includes calculation formulas. Primer-BLAST - Provides primers specific to the PCR template sequence. Includes primer pair specificity checking against a selected database. StarORF - A Java application that facilitates the identification of potential ORFs, based on provided DNA sequence and probable ORF length. ORF Finder - Identifies all ORFs using the standard or alternative genetic codes. Provides an interactive, graphical interface and allow for saving of a deduced amino acid sequence in various formats. Allows searching against the sequence database using the BLAST server. REBsites - Provides restriction enzyme digest analysis of the input DNA sequence. Results are presented graphically as a "virtual gel". TACG - DNA restriction enzyme analysis. Provides a number of hits per enzyme and a list of non-cutters. Allows for selection of the enzymes and parameters. Silent - Silent mutation restriction enzyme scan. Finds new restriction sites to insert (mutate) with no translation change. RaCC: Rare Codon Calculator - Determines the number and highlights a rare E. coli codons in a provided DNA sequence. Maintained by UCLA, USA. Rare Codon Analysis - Provides graphical and numerical representation of the codon adaptation index, frequency distribution and GC content of the DNA sequence. Maintained by the GenScript, USA.