Ingenetix GmbH - Develops technology and products for DNA and mRNA research. Also provide DNA testing for the determination of parentage/paternity and custom DNA sequencing, oligonucleotide synthesis, genotyping services, pharmacogenetics and quantitative PCR, in Vienna, Austria. Roe Laboratory Protocols - Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK. The Web Guide of PCR - List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina. HiFi DNA - HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails. PCR Protocols - Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University Thermostable DNA Polymerases - Discussion of their origin and briefly their properties. From the web site of Colorado State University. Which DNA Marker for Which Purpose? - Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Göttingen. Single Tube Confirmation PCR Protocol - For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA. GenHunter - Manufacturer of material for differential display PCR in Nashville, Tenn USA. Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation - Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA. T-DNA Generated Enhancer Traps in Arabidopsis - Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH. PCR Amplification of cDNA Segments by 2 Stage Nested PCR - Protocol from the method database of NIH, USA. PCR-ELISA and Related - P Zhang, CJ Gebhart, D Burden, GE Duhamel: A low technology alternative to real time PCR, technical article on the site of BT&C, Inc Bridgewater, NJ, USA. Nematode ITS1 Size Variation - Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes, from University of Nebrasca. Attotron Biosensor Corporation - Research and development company for development of biosensors and related products for the research and educational markets. Protocols Online: PCR Protocols - Extensive collection of PCR protocols and methods from Protocol On Line. Kary B. Mullis - Autobiography - The originator of PCR, from the Nobel e-museum web site. Reference in PCR - Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers. GeneOhm Sciences - Tests on group B Streptococcus and methicillin resistant Staphylococcus aureus by PCR / DNA sequencing. Principle of PCR - Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium. PCR Animated - Animation illustrating the principle of PCR, from the University of Ghent, Belgium. PCR Gateway - A directory of PCR techniques, PCR protocols, PCR troubleshooting, PCR websites and online resources from the publisher Horizon Press. BioRad, Amplification, PCR - Division of BioRad Laboratories that manufactures and sells instruments for PCR, in Hercules, California, USA. PCR Guru - A downloadable textbook on PCR setup and optimization, not free. The PCR Encyclopedia - Describes plans for a free encyclopedia dedicated to the polymerase chain reaction (PCR). Primerfox - Free online tool for generation of PCR primers. PrimerDigital - International biotechnology company specialized in a design service for PCR primers and probes, PCR-based technology development, projects for development of polymorphism and software development. Inverse PCR for Use with Snyder mTn-lacZ/LEU2-based Mutagenesis - Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA. Inverse PCR Protocol - Step by step protocol, from the web site of the Department of Biology, University of Michigan, USA. PCR Protocol - Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA. Rational Primer Design Greatly Improves Differential Display-PCR (DD-PCR) - Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.